Correlative Microscopy
The SECOM platform integrates fluorescence microscopy and SEM

Correlative Light and Electron Microscopy (CLEM) - Scanning Electron Combined Optical Microscope (SECOM) -
SECOM Principle
- SECOM Configurations - Wide-Field Fluorescence Microscope - Confocal Laser Scanning Microscope

Correlative light and electron microscopy (CLEM)

Correlative Microscopy
Correlative microscopy is the integration of multiple imaging and analysis techniques, with the aim of increasing efficiency in workflow, time to results and overall knowledge of the sample under study. Correlative microscopy is not a new idea, but in the recent years has become more important as researchers realize the benefits of bridging the gaps with a combine platform and providing answers neither instrument could provide on it’s own.

Correlative Light and Electron Microscopy (CLEM)
In life sciences, the two most productive techniques are fluorescence microscopy and electron microscopy. Correlative light and electron microscopy, commonly referred to as CLEM, combines the functional information from fluorescence microscopy with the structural information of the electron microscope.

Scanning Electron Combined Optical Microscope (SECOM)
The SECOM platform is a unique correlative microscopy solution. The SECOM platform integrates a fluorescence microscope with a scanning electron microscope (SEM).  This combination makes it possible to do fast correlative microscopy with high overlay precision. The system is easy to use for both optical and electron microscopists and makes correlation as simple as drag-and-drop.

example correlative microscopy: Fluorescence with SEM

SECOM Principle
The SECOM platform integrates the functionality of a fluorescence microscope into the chamber of a scanning electron microscope. In a single device, the SECOM platform allows the user to obtain functional color information through fluorescence microscopy and structural information by using the scanning electron microscope. With SECOM, the optical axes of both microscopes are precisely aligned; the overlay of the fluorescent and electron images becomes as easy as drag-and-drop.

SECOM Configurations
The SECOM platform is configurable based on your labs experimental needs. The platform can be purchased as a wide-field or confocal configuration. Both configurations are compatible with new or installed scanning electron microscopes.

Wide-Field Fluorescence Microscope
This product combines a wide-field epi-fluorescence microscope with the SECOM platform. This combination serves to obtain a direct broad overview of the sample. It comes with navigation software, which allows for obtaining electron microscopy images based on the optical microscope, thus minimizing specimen damage.

Confocal Laser Scanning Microscope
The CLSM combines a Nikon C2+ scan head with the SECOM platform. The use of a confocal microscope makes it possible to obtain an image stack for 3D image reconstruction. The confocal microscope gives high-resolution images, giving optimal electron-optical overlay.

Please contact Nanounity for more information on the SECOM product and correlative microscopy solutions at or feel free to call us at (408) 235-8888.